FIG. 2.

Inhibition of HIV-1, SIVmnd1, and SIVtan infection by expression of hA3G (A) or hA3F (B) in 293T cells. Subconfluent 293T monolayers (35 mm in diameter) were cotransfected with 3 μg of wild-type (wt) or Δvif provirus and 0, 3, or 9 μg of pAPOBEC3G or pAPOBEC3F; the DNA content of each transfection cocktail was increased to 12 μg, using a control vector. At ∼24 h, culture supernatants were harvested and normalized by RT content, and virus infectivities were determined using C8166-CCR5/HIV-CAT cells. Values are presented as percentages of infectivity relative to that of virus produced in the absence of hA3G or hA3F, and error bars show standard deviations.