FIG. 3.
Effect of HIV and SIV Vif proteins on replication of HIV-1, SIVmnd1, or SIVtan in T cells. (A to D) HUT78-CCR5 or Jurkat-CCR5 cells stably transduced with HIV-1IIIB vif, HIV-1IIIB Δvif, or SIVtan1 vif were infected with normalized wild-type (wt) or Δvif virus stocks and maintained for up to 4 weeks at 0.25 × 106 to 2 × 106 cells per ml. Virus replication was monitored by measuring supernatant levels of p24Gag or RT. (E) Jurkat cells express hA3F. An ethidium bromide-stained agarose gel shows the products of PCRs performed using primers specific for hA3G or hA3F (5). The templates were either cDNA generated from mRNA isolated from the indicated T cell lines (lanes 3 to 5), water as a negative control (lane 2), or plasmids encoding hA3G or hA3F as positive controls (lane 1).