Bactericidal activity of recombinant α-defensins. (A) Primary sequence alignment for each of the peptides showing the tridisulfide array and the position of three invariant residues (boxed region). (B) AU-PAGE of recombinant α-defensins. Lane 1, Crp4; lane 2, Crp3; lane 3, RMAD-3; and lane 4, RMAD-4. Representative bactericidal activities of recombinant RMAD-3 (hexagon), RMAD-4 (diamond), and HD5 (square) against E. coli (C), V. cholerae (D), L. monocytogenes (E), and S. aureus (F) are illustrated.