Figure 3.

hkt1-4 suppresses NaCl sensitivity of sos3-1 plants but causes NaCl sensitivity of wild-type (gl1) plants. Stratified seeds in soil were transferred to a growth chamber for germination and plant growth. Plants were irrigated by placing the pots in a container of EXCEL solution until the soil was saturated. EXCEL includes 167 mg L⁻¹ K⁺, 67 mg L⁻¹ Ca²⁺, and no detectable Na⁺, according to the manufacturer's specifications. One-month-old plants were transplanted into Turface. After 1 week of acclimation, plants were watered every 3 or 4 d with EXCEL solution (0 mm NaCl) or supplemented with NaCl (50 mm NaCl for four treatments followed by 100 mm NaCl for 7 treatments). Photographs were taken 5 weeks after beginning of treatment.