Figure 2.

Patch-clamp recordings of K⁺ channel currents in cells obtained from the apex of roots of Na⁺-sensitive and K⁺-channel mutants. A, Representative recordings of whole-cell currents obtained from cells that were voltage clamped at 20 mV steps between −180 mV and +120 mV. Only the akt1 mutant displayed abnormal inward (negative) currents, which are here plotted downward. B, Steady-state inward K⁺ current plotted versus the clamped membrane potential. Shown are the average steady-state values ± SEM obtained for five cells of each genotype. The sos and skor mutants were not different than wild type, but akt1 showed its characteristic absence of inward current.