Figure 1.

Nucleotide sequence (GenBank accession no. AY496702) of the PIMT2 gene and its deduced amino acid sequence. Nucleotide and amino acid numbers are indicated on the left and right, respectively. The putative ABRE motif in the promoter is identified as such and is in bold type. The transcription initiation site (TIS) is demarcated with an arrow. Italicized, lowercase sequence denotes the 5′- and 3′-UTRs, with a polyA signal in the latter underlined. Introns are designated by lowercase letters. The 9-nt alternative 3′ splice site is boxed and the Hpy188 III recognition site used in CAPS is in bold text. The predicted NLS in the amino acid sequence is boxed. Amino acids in common among Arabidopsis PIMT1, PIMT2, and PIMT from wheat (GenBank accession no. Q43209) are in black boxes. The three regions conserved among different families of methyltransferase utilizing S-AdoMet as a cosubstrate are labeled and highlighted in black. The two regions that appear to be unique to protein-l-iso-Asp (d-Asp) methyltransferase are also highlighted in black and labeled pre-region I and post-region III.