Figure 8.

Qualitative multiplex RT-PCR analysis of RNA transcripts extracted from stressed or unstressed, 14-d-old seedlings. A, RNA was extracted from seedlings on water, after incubation on ABA, or following exposure to drought or salt stress. The RT-PCR products were obtained as described in Figure 4A. B, Amplicons of PIMT2 were analyzed as in Figure 4B. C, A separate experiment on a different set of seedlings was conducted and amplicons were separated on 3.5% acrylamide nondenaturing gels at 25mA constant current. Gels were stained for 20 min in SYBER-Gold (Molecular Probes), visualized, and quantified (data not shown) for comparison with values obtained from the first set of stressed seedlings using CAPS.