Table I.
Construct | Fatty Acid Composition
|
|||||||
---|---|---|---|---|---|---|---|---|
16:0 | 18:0 | 20:0 | 22:0 | 24:0 | 26:0 | LCFA | VLCFA | |
% (w/w) of total fatty acids [% increase]a | ||||||||
RD | 20.38 ± 0.12 | 7.99 ± 0.26 | 1.32 ± 0.03 | 0.59 ± 0.03 | 0.70 ± 0.03 | 0.89 ± 0.16 | 96.28 ± 0.31 | 3.72 ± 0.31 |
SF | 18.01 ± 0.42 | 5.23 ± 0.41 | 1.58 ± 0.54 [19.7] | 1.32 ± 0.16 [123.9] | 1.93 ± 0.27 [175.7] | 1.31 ± 0.20 [147.2] | 91.37 ± 0.84 | 8.63 ± 0.84 [131.9] |
SMF | 19.48 ± 0.34 | 7.12 ± 0.19 | 1.30 ± 0.02 | 0.57 ± 0.03 | 0.73 ± 0.04 | 1.01 ± 0.32 | 95.59 ± 0.40 | 4.41 ± 0.40 [18.5] |
Results represent the average (±se) of 10 measurements using independent calli. Constructs: RD, Control (plasmid-only) transgenic calli; SF, 35S: nasturtium FAE transgenic calli; SMF, 35S: mutated nasturtium FAE transgenic calli.
Relative to value for calli from RD, the tobacco control (plasmid-only) calli, set at 100%.