Table II.
Fatty acid composition of transformed tobacco leaves
| Construct | Fatty Acid Composition
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 16:0 | 18:0 | 18:3 | 20:0 | 20:1Δ11 | 22:0 | 24:0 | LCFA | VLCFA | |
| % (w/w) of total fatty acids [% increase]a | |||||||||
| RD | 16.32 ± 0.14 | 3.94 ± 0.11 | 53.30 ± 0.72 | 0.53 ± 0.02 | 1.18 ± 0.00 | 0.27 ± 0.01 | 2.74 ± 0.09 | 93.77 ± 0.29 | 6.23 ± 0.29 |
| SF | 15.83 ± 0.14 | 3.35 ± 0.12 | 47.02 ± 0.66 | 0.91 ± 0.12 [71.7] | 2.34 ± 0.12 [98.3] | 0.42 ± 0.02 [55.6] | 4.14 ± 0.15 [51.1] | 88.64 ± 0.35 | 11.36 ± 0.35 [82.3] |
| SMF | 15.53 ± 0.17 | 4.00 ± 0.12 | 47.25 ± 0.85 | 0.98 ± 0.16 [84.5] | 2.61 ± 0.02 [121.2] | 0.30 ± 0.01 | 3.24 ± 0.08 [18.2] | 90.05 ± 0.28 | 9.95 ± 0.28 [59.7] |
Results represent the average (±se) of 10 measurements using leaf discs from 10 independent transgenic plants. Constructs: RD, Control (plasmid-only) transgenic leaves; SF, 35S: nasturtium FAE transgenic leaves; SMF, 35S: mutated nasturtium FAE transgenic leaves.
a
Relative to value for leaves from RD, the tobacco control (plasmid-only) plants, set at 100%.