Figure 5.

Determination in pea leaf peroxisomes of NO^˙ production from l-Arg (NOS activity) by ozone chemiluminiscence. Reaction mixtures containing peroxisomal fractions were incubated in the absence and presence of l-Arg (1 mm), NADPH (1 mm), EGTA (0.5 mm), calmodulin (10 μg/mL), BH₄ (10 μm), antibody against iNOS, 200 μm CM, 0.01% AAN, and 1 mm azide. Then the NO^˙ production was assayed using a 1 mm l-Arg concentration and an incubation time of 30 min. The NO^˙ produced was quantified by ozone chemiluminiscence using a nitric oxide analyzer. Results are means of samples from at least three different Suc-density gradients.