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. 2016 Dec 22;8(1):1–10. doi: 10.1016/j.stemcr.2016.11.011

Figure 1.

Figure 1

Vitamin C and l-Proline Induce Opposite Effects on DNA Methylation in ESCs

(A) Schematic representation of the time-course colony assay. FBS/LIF ESCs plated at low density ± VitC (500 μM)/± l-Pro (150 μM) for 5 days (left panel). Representative pictures of crystal violet-stained colonies (right panels). Scale bars, 250 μm.

(B) Time-course mass spectrometry (MS) analysis of 5hmc and 5mc levels in ESCs ± VitC (500 μM)/± l-Pro (150 μM). Data are relative levels (%) compared with day 1.

(C) Heatmap (CIMminer, http://discover.nci.nih.gov/cimminer/) showing the differentially methylated regions (DMRs) in ESCs ± VitC (500 μM)/± l-Pro (150 μM) at days 2 and 5.

(D) Pie graphs showing the percentage of DMRs shared by VitC- and l-Pro-treated ESCs at days 2 and 5.

See also Figure S1.