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. Author manuscript; available in PMC: 2018 Jan 9.
Published in final edited form as: Cancer Cell. 2017 Jan 9;31(1):50–63. doi: 10.1016/j.ccell.2016.12.002

Figure 2. Chemical screen of 21 epigenetic probes identifies SETD8 inhibitor as one of the top compounds with the lowest IC50.

Figure 2

(A) Flow chart of the chemical screen: 21 epigenetic probes were tested in the indicated cell lines and images were recorded by an IncuCyte Zoom System in order to calculate IC50.

(B) Heatmap showing the average IC50 of the indicated epigenetic chemical probes at 96 hr in 4 MYCN-WT and 4 MYCN-amp NB cell lines compared to 2 control cell lines (ARPE-19 and 293T). The color key represents the average IC50 (μM) values: red color indicates low IC50 whereas purple color represents high IC50. Cell confluence was recorded by an IncuCyte Zoom System. The average IC50 was calculated based on the cell confluence values of 3 biological replicates across 8 NB cell lines at 96 hr using GraphPad Prism. For in vitro therapeutic index (IVTI) and p values, see Figure S2 and Table S4.

(C) In vitro UNC0379 treatment of NB cell lines and control cell lines ARPE-19 and 293T (average of 3 biological replicates).

(D, E) A representative experiment showing effects of in vitro treatment of SK-N-AS cells with UNC0379 at indicated times (D) and concentrations (E). Bars show the average of 3 replicates ± SD. In panel (D) the images were captured by IncuCyte Zoom. Scale bars, 300 μm.

See also Table S4 and Figure S2.