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Two-dimensional gel electrophoresis of different cellular fractions from D. salina: A, cPm; B, cyt soluble; C, chl soluble. Left, Proteins from 0.5 m NaCl cells; right, proteins from 3 m NaCl cells. Polypeptides (250 μg) were extracted and separated on 18-cm IPG strips and then applied on 11% SDS-PAGE. Gels were stained with Coomassie Brilliant Blue R-250. Numbers indicate the selected spots and refer to the numbers in Table I; the circled spots are unidentified spots.
