Figure 1.

Expression of SNEV Is Induced during Adipogenesis

(A) SNEV mRNA expression in hASCs at different days post-induction of adipogenesis was quantified by RT-qPCR. Data points represent averages from three independent differentiation experiments (donors 803, 812, and 851). Error bars indicate SD. Two-way ANOVA p value of 0.045.

(B) Whole-cell lysate of differentiating (D) and undifferentiated (U) hASCs were submitted to western blotting and probed with anti-SNEV antibody. Anti-GAPDH was used to ensure equal loading. Numbers represent intensities of bands normalized to GAPDH. Upper panel, exemplary western blot. Lower panel, the graph shows average of band intensities normalized to GAPDH derived from three independent differentiation experiments (donors 803, 812, and 851). Error bars indicate SD. Two-way ANOVA p value of 0.032.

(C and D) Adipogenic differentiation was confirmed by qPCRs against adipogenic marker genes PPARγ and FASN. Data points represent the average from three independent differentiation experiments (donors 803, 812, and 851). Error bars indicate SD. Unpaired two-sided Student's t tests were performed to compare differentiated and undifferentiated samples at the same time points: ^∗∗∗∗p < 0.0001, ^∗∗p < 0.01.

(E) Differentiated (D) and undifferentiated (U) hASCs of donor 812 were stained with anti-SNEV antibody. Scale bar, 25 μm.