Characterization of piPSCs by Immunofluorescence Microscopy
(A) Translocation of Rex1 from cytoplasm to nuclei in piPSCs induced by epigenetic factors.
(B) piPSCs induced by leukemia inhibitory factor without basic fibroblast growth factor express SSEA-1.
(C) Expression of pluripotent marker genes, Oct4, Nanog, and Sox2 in nuclei of piPSCs. Scale bars, 10 μm (A) or 20 μm (B) and (C). See also Figure S3.