Figure 3.

Differentiation Potential of piPSCs Induced by Addition of Epigenetic Factors

(A) Left, differentiation in vivo of piPSCs by teratoma formation test following injection into non-obese diabetic/severe combined immunodeficiency mice. Right, H&E staining of teratoma tissues derived from piPSCs. ^∗, neural epithelium (ectoderm), ^∗∗, muscle (mesoderm), ^∗∗∗, gland epithelium (endoderm).

(B) Weight and time of teratomas formed from piPSCs.

(C) Immunofluorescence of the teratomas showing markers representative of three germ layers, nestin (ectoderm), smooth muscle actin (SMA, mesoderm), and alpha 1-fetoprotein (AFP, endoderm). Scale bars, 100 μm (A) or 50 μm (C). See also Figure S3.