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Tricine-SDS-PAGE analysis of BrCN digestion peptides of CYP3A4 variants labelled with DyLight 549 maleimide 10. The image was acquired with a Typhoon Trio + scanner using the Green-excited mode (λex = 532 nm), with a 580 nm band-pass filter (580 BP 30, transmits light between 565 and 595 nm with a transmission peak centered on 580 nm). Mutations include: C98S for mt 1, C98S/C377S/C468G for mt 2, C98S/C239S/C468G for mt 3, and C64S/C98S for mt 4.
