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. Author manuscript; available in PMC: 2017 Jan 12.
Published in final edited form as: Cell Metab. 2015 Jun 2;21(6):883–890. doi: 10.1016/j.cmet.2015.05.016

Figure 3. AMPK-MKO Mice Have Impaired Autophagic Flux in Response to Fasting.

Figure 3

(A and B) Photomicrographs (A, scale bar, 10 μm) and quantification (B, n = 3–4) of RFP-LC3 puncta in TA muscle.

(C and D) LC3II/LC3I expression in mice treated with sterile water or colchicine (C, n = 9–19) and Parkin expression (D, n = 4–5).

(E and F) Photomicrographs of Parkin (scale bar, 10 μm), Tom20, and merged image (scale bar, 1 μm) (n = 3 per group) (E) and quantification in TA muscle (F).

Data are means ± SEM. * WT versus AMPK-MKO p < 0.05, **p < 0.01, ***p < 0.001, ### fed versus fasted p < 0.001, Φ Φ Φ fast saline versus fast colchicine p < 0.001.