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. Author manuscript; available in PMC: 2017 Jan 12.
Published in final edited form as: Diabetes. 2016 Aug 23;65(11):3352–3361. doi: 10.2337/db16-0564

Figure 2.

Figure 2

Salicylate uncouples primary hepatocytes, reduces mitochondrial membrane potential, and increases mitochondrial proton conductance. A: Representative oxygraph trace in permeabilized primary hepatocytes illustrates the change in oligomycin-insensitive respiration (state 4o) during salicylate titration. B: Quantification of the increase in state 4o respiration when titrating salicylate (Sal). C and D: Change in TMRM fluorescence in response to salicylate in permeabilized primary hepatocytes. FCCP, carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone. E: Proton current at a given membrane potential in the presence or absence (control) of 1 mmol/L salicylate. F: Proton conductance alterations with salicylate titration. Data are expressed as mean ± SEM (n = 3–6). *P < 0.05 by one-way ANOVA with Bonferroni post hoc test or two-tailed Student t test.