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. Author manuscript; available in PMC: 2017 Jan 12.
Published in final edited form as: Diabetes. 2016 Aug 23;65(11):3352–3361. doi: 10.2337/db16-0564

Figure 5.

Figure 5

Salsalate does not alter BAT thermogenesis in vivo, but salicylate increases UCP1-inhibited BAT mitochondrial respiration in vitro. A and B: VO2 response to CL-316,243 (0.033 nmol/g body weight) in mice fed the HFD or the HFD with salsalate supplementation (HFD+SAL). C and D: BAT thermogenesis in response to CL-316,243 in HFD and HFD+SAL mice. E: Representative trace of salicylate-driven BAT mitochondrial respiration in the presence of 2 mmol/L GDP. AmA, antimycin A; LCarn, L-carnitine; P-CoA, palmitoyl-CoA. F: Quantification of the increase in respiration when titrating salicylate. Data are expressed as mean ± SEM (n = 5–9 for in vivo data and n = 6 for BAT mitochondrial respiration experiments). #P < 0.05 by two-way ANOVA with Bonferroni post hoc test compared with saline and *P < 0.05 by one-way ANOVA were used to detect statistical differences.