Table 1.

EcPanK kinetic parameters.

	Km (μM)	kcat(s−1)	kcat/Km (mM−1 s−1)
Pantothenate (1)
exp	21 ± 3	1.07 ± 0.05	50 ± 7
lit6	34 ± 6	0.76 ± 0.03	21 ± 2
Pantetheine (2)
exp	60 ± 10	1.03 ± 0.06	17 ± 3
lit17	91 ± 10	0.32 ± 0.02	3.5 ± 0.4
N5-Pan (3)	36 ± 3	0.93 ± 0.04	26 ± 2
4	120 ± 10	1.12 ± 0.05	10 ± 1
5	1200 ± 400	0.13 ± 0.03	0.1 ± 0.1
6	NA[a]
7	NA
8	190 ± 20	0.96 ± 0.04	5.1 ± 0.6
9	1000 ± 200	0.49 ± 0.06	0.5 ± 0.1
10	700 ± 300	0.23 ± 0.05	0.3 ± 0.2
11	NA
12	240 ± 30	1.09 ± 0.07	4.5 ± 0.6
34	33 ± 4	1.24 ± 0.06	38 ± 5
35	>1000	1.19 ± 0.11	0.5 ± 0.1
36	>1000	0.75 ± 0.18	0.3 ± 0.1
37	350 ± 20	1.03 ± 0.05	2.9 ± 0.3
38	43 ± 4	1.02 ± 0.05	24 ± 3
39	67 ± 6	1.15 ± 0.05	17 ± 2
40	32 ± 3	1.13 ± 0.05	35 ± 4
41	22 ± 7	0.46 ± 0.03	21 ± 6
42	50 ± 7	1.02 ± 0.05	20 ± 3
43	130± 30	0.94 ± 0.08	7 ± 2
44	39 ± 5	0.78 ± 0.04	20 ± 3
45	110 ± 10	1.15 ± 0.06	11 ± 1
46	450 ± 50	1.44 ± 0.09	3.2 ± 0.4
47	NA
48	NA
49	200 ± 100	0.45 ± 0.09	1.9 ± 0.9
50	400 ± 200	0.18 ± 0.05	0.4 ± 0.2
51	NA
52	NA
62	230 ± 20	1.17 ± 0.06	5.1 ± 0.5
63	1200 ± 400	0.28 ± 0.06	0.2 ± 0.1
64	590 ± 60	0.89 ± 0.06	1.5 ± 0.2
65	110 ± 10	1.04 ± 0.06	9 ± 1
66	NA
67	NA

^[a]NA – no observable activity up to 1 μM