Table II.

Contribution of Pro and K⁺ to the altered solute content of lwr1 and lwr2

	Agar
	Ben		lwr1 ψw		lwr2
	−0.25	−0.75	−0.25	−0.75	−0.25	−0.75
	MPa
ψs100 (MPa)	−0.428	−0.661	−0.502	−0.803	−0.396	−0.503
Solute content (mm)a	175	270	205	328	162	206
Solute difference (mm)b			30	58	−13	−64
Pro100 (mm)c	0.31	3.20	0.76	3.86	0.49	2.03
Pro difference (mm)			0.45	0.66	0.18	−1.17
% Solute differenced			2	1	0	2
K100+, (mm)e	59	108	61	136	55	86
K+ difference (mm)			2	29	−4	−22
% Solute differencef			7	50	26	34

ψ_s100 (Fig. 5) was converted to solute concentration (mm) and used to calculate the difference in solute concentration between mutants and wild type at −0.25 and −0.75 MPa. Pro (Fig. 4) and K⁺ (Fig. 6) contents were adjusted to 100% RWC and used to calculate the difference in content of Pro and K⁺ relative to wild type. These values were then compared to the total difference in solute content between mutant and wild type to estimate the contributions of Pro and K⁺ to the altered solute content of the mutants.

^aTotal solute content at 100% RWC calculated using the equation: ψ_s = −RTC.

^bDifference in total solute content at 100% RWC between the mutant and wild type (Ben); negative values for lwr2 indicate that wild-type solute content was greater than that of lwr2.

^cPro content at 100% RWC calculated from data in Figures 4 and 5; a correction for percent dry weight (≤4%) was applied to the Pro data.

^dPercent of the total difference in solute content at 100% RWC that can be accounted for by altered Pro content.

^eK⁺ content at 100% RWC calculated from data in Figures 5 and 6.

^fPercent of the total difference in solute content at 100% RWC that can be accounted for by altered K⁺ content.