Figure 5.

CDKn1c Has an Unannotated Isoform in Mouse HSCs in Addition to the Canonical One

(A) Raw RNA-seq data showing two known isoforms (from Ensembl). Raw reads and junctions are from the IGV browser. Note that the expressed variant isoform is unannotated according to the Ensembl or RefSeq databases. The enlarged area demonstrates multiple reads from a part of the sequence thought to be part of an intron.

(B) A Sashimi plot of CDKn1c. The read number is represented by the height of the schematic; the connecting arcs represent splice junctions. The data were taken from four samples (A0, B0, B1, and A3, in descending order, respectively).

(C) Histogram chart of the expression of each isoform relative to Hprt. Data of technical triplicate average ± SD from one representative experiment out of four are shown.