Figure 3.

Differentiation Alters the Ability of GBM Stem Lines to Repair DNA Damage Caused by Ionizing Radiation

(A) Western blots probed for PRKDC or XRCC5/XRCC6 (Ku80/Ku70) in GSC grown in NB medium or NB-BMP4-FBS. β-Actin or Vinculin was a loading control. HeLa included as positive control and NHA for comparison.

(B) Representative images of RAD51 foci in the nuclei of GSCs grown in either NB medium or NB-BMP4-FBS, 4 hr after treatment with 3 Gy radiation.

(C) Quantification of RAD51 foci from (B) (n = 3 independent experiments with ≥100 cells counted per condition).

(D) Representative images of GSC nuclei stained for TP53BP1 foci 30 min post-treatment with 3 Gy radiation.

(E) Quantification of TP53BP1 foci from (E) (n = 6 independent experiments with ≥100 cells counted per condition).

(F) Representative images of GSC nuclei stained for γH2AX 24 hr following 3 Gy radiation.

(G) Quantification of γH2AX foci from (F) (n = 3 independent experiments with ≥100 cells counted per condition).

Error bars indicate SEM. Statistical significance was calculated using one-way ANOVA with ^∗∗∗p < 0.001. Scale bars, 20 μm.