Skip to main content
. 2017 Jan 10;8(1):69–83. doi: 10.1016/j.stemcr.2016.12.008

Figure 2.

Figure 2

Synaptic Integration of Nrl-GFP/ROSA::Nrl-CtBP2-tdTomato Graft Retina into L7-GFP Host Mice

(A) GFP-positive bipolar cells extend their dendrites through graft INL to reach CtBP2-tdTomato on graft ONL (DD78).

(B) CtBP2-positive presynaptic terminals contact the tips of rod bipolar dendrite terminals in L7-GFP wild-type retina. Front view image of the section between the yellow dashed lines in side view, and side view image of the section between the yellow dashed lines in front view are presented.

(C–C‴) 3D observation of contact between GFP-positive host bipolar cells and CtBP2-tdTomato in the graft ONL (DD35) with (C′) and without (C) DAPI nuclear stainings, with front (C″) and side slice views (C‴). (C″) is the image of the section between the yellow dashed lines in (C‴), and (C‴) is the image of the section between the two vertical lines in (C″).

(D) CACNA1s localizes at dendritic tips of PKCα-positive dendrite terminals in a wild-type retina.

(E and E′) CtBP2-tdTomato in the graft ONL (DD78) are coupled with CACNA1s (arrows) at the tips of L7-GFP-positive host bipolar cells as shown in the side and bottom sliced views.

Scale bars, 20 μm (A, C), 10 μm (B), and 5 μm (D, E).