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RT-PCR analysis of NTHK2 expression upon various treatments. Tobacco seedlings were subjected to dehydration, ABA, CaCl2, and NaCl treatments for the indicated times, and total RNA was used for RT-PCR analysis. For dehydration treatment, only roots were used for analysis. Tobacco actin genes were amplified as a control. The amplified products of NTHK2 and actin are 415 bp and 600 bp, respectively.
