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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2016 Dec 6;28(2):389–392. doi: 10.1007/s13361-016-1552-4

Figure 1.

Figure 1

(A) Mass spectra from intact measurements of FPOP-labeled WT SOD1 (+16 charge) obtained by adjusting [His]. (B) Intact protein fraction modified vs. reporter fraction modified under various scavenging conditions. The resulting curve is independent of the presence of FPOP adventitious scavengers, it demonstrates that incorporation of reporter peptide could normalize (or correct for) the FPOP outcome, making possible unbiased comparison between one state of protein and another (e.g., apo and holo).