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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Connect Tissue Res. 2016 Jul 12;58(1):64–75. doi: 10.1080/03008207.2016.1211113

Figure 1. IL-1β- induced OA cartilage degradation was suppressed by SAHA in vitro.

Figure 1

(a) Cartilage explants were carefully dissected and cultured in CellGro ACTive medium and treated with IL-1β in the presence or absence of 1μM SAHA for the indicated time points. Supernatant was collected and GAG content was measured by a commercially available kit (#8000, Astarte Biologics). (b) and (d) Supernatant from control and treated cartilage explants was used to perform the Western Blotting using anti-MMP-13 (#sc-30073, Santa Cruz Biotechnology) and anti-IL-6 (#12153, Cell Signaling) antibody. (c) Control or treated cartilage explants were used to isolate total RNA and TaqMan assay was employed to quantify the expression of IL-6 mRNA. Bars represent ±SD of independent experiments using five patients’ cartilage samples (*P <0.05; **P <0.005; paired student t-test).