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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Connect Tissue Res. 2016 Jul 12;58(1):64–75. doi: 10.1080/03008207.2016.1211113

Figure 5. Transcriptional activation of MCPIP1 by SAHA in OA chondrocytes.

Figure 5

(a) OA chondrocytes were stimulated with IL-1β (2ng/ml) in the presence or absence of SAHA (1μM) for 16 hrs. Chondrocytes were harvested and nuclei were isolated and subjected to nuclear run on assay as described above. Total RNA was isolated and MCPIP1 expression was quantitated using TaqMan assay (n=3). (b) OA chondrocytes were transfected with full length or deletion mutants of the MCPIP1 promoter reporter constructs and were stimulated with IL-1β alone or IL-1β + SAHA. Luciferase activity was measured after 24 hrs of stimulation. Renilla luciferase was used to normalize the transfection efficiency. (c) Diagramatic representation of the the156bp MCPIP1 promoter fragment with predicted transcription factors binding sites. (d) Transcription factor CEBPα was upregulated upon IL-1β and SAHA treatment determined by TaqMan assay and Western blot analysis. (*P <0.05; **P <0.005; paired student t-test).