Table 2.
Supplementation with Schneider medium prolongs viability with sustained and higher transepithelial transport
| Solution | V t (mV) | R t (Ω cm2) | I sc (μA cm–2) | V b (mV) | V a (mV) | R a/R b | |||
|---|---|---|---|---|---|---|---|---|---|
| A | L | Control‐R | −43.6 ± 6.4 | 1189 ± 229 | 36.9 ± 9.0 | −65.8 ± 6.7 | 22.2 ± 6.0 | 0.36 ± 0.11 | |
| B | Control‐R | (n = 11) | (n = 8) | (n = 8) | (n = 11) | (n = 11) | (n = 8) | ||
| L | Control‐R + 0.1% DMSO | −41.7 ± 5.4 | 1178 ± 260 | 35.4 ± 10.8 | –70.6 ± 5.7 | 28.9 ± 5.7 | 0.42 ± 0.2 | ||
| B | Control‐R + 0.1% DMSO | (P > 0.5) | (P > 0.8) | (P > 0.5) | (P > 0.8) | (P > 0.5) | (P > 0.5) | ||
| B | L | Sch‐R | −46.9 ± 1.6 | 672 ± 43 | 65.8 ± 5.6 | −64.8 ± 2.6 | 17.9 ± 2.3 | 0.44 ± 0.16 | |
| B | Sch‐R | (n = 44) | (n = 44) | (n = 44) | (n = 44) | (n =44) | (n = 44) | ||
| L | Sch‐R | −46.0 ± 2.3 | 760 ± 40 | 57.4 ± 3.7 | −70.3 ± 5.5 | 24.3 ± 6.2 | 0.65 ± 0.24 | ||
| B | Control‐R | (P > 0.4) | (P > 0.07) | (P > 0.1) | (P > 0.4) | (P > 0.3) | (P > 0.08) |
Values for perfused midgut, both ends cannulated and electrically sealed from bath. B, bath solution; L, lumen solution; Sch‐R, Schneider Ringer. I sc, short circuit current; R a, apical membrane resistance; R b, basal membrane resistance; R t, transepithelial resistance; V a, apical membrane potential; V b, basal membrane potential; V t, transepithelial potential at perfusion end.