. 2004 Oct;136(2):3058–3069. doi: 10.1104/pp.104.047506

Table II.

Free amino acid contents in pig1-1 and wild-type (Ws-2) plantlets grown on nonsupplemented MS and MS + 2 mm Phe agar plates

Amino Acid							Amino Acid Ratio on MS	Amino Acid Ratio on 2 mm Phe
Amino Acid	MS		MS + 2 mm Phe		MS + 2 mm Phe		Fold Increase in pig1-1⁺	Fold Decrease in pig1-1⁺	Repression Ratio*
	Ws-2	pig1-1	Ws-2	pig1-1	Ws-2	pig1-1	pig1-1/Ws-2	Ws-2/pig1-1	pig1-1/Ws-2
	nmol g fw⁻¹				mol %
Glu	250 ± 11	403 ± 26^a	580 ± 35	336 ± 25^a	2.6 ± 0.1	3.7 ± 0.5^a	1.6	1.7	2.8
Gln	1,207 ± 61	2,650 ± 345^a	9,372 ± 933	3,251 ± 458^a	38.6 ± 1.3	34.8 ± 0.7^a	2.2	2.9	6.3
Asp	146 ± 9	223 ± 11^a	296 ± 8	208 ± 10^a	1.4 ± 0.1	2.3 ± 0.3^a	1.5	1.4	2.2
Asn	624 ± 65	1,132 ± 164^a	3,427 ± 353	1,379 ± 157^a	15.3 ± 0.3	14.9 ± 0.4	1.8	2.5	4.5
Ser	457 ± 13	665 ± 33^a	785 ± 35	684 ± 19^a	3.6 ± 0.3	7.6 ± 0.9^a	1.5	1.1	1.7
Gly	430 ± 27	756 ± 80^a	717 ± 85	459 ± 9^a	3.2 ± 0.2	6.5 ± 0.5^a	1.8	1.6	2.7
Ala	147 ± 4	203 ± 16^a	452 ± 37	177 ± 3^a	2.0 ± 0.1	2.3 ± 0.1^a	1.4	2.6	3.5
Cys	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
His	16 ± 1	23 ± 3^a	147 ± 9	53 ± 9^a	0.7 ± 0.03	0.6 ± 0.03^a	1.5	2.8	4.0
Arg	777 ± 49	635 ± 18^a	760 ± 56	719 ± 37^a	3.4 ± 0.2	7.9 ± 0.5^a	0.8	1.1	0.9
Pro	162 ± 13	375 ± 48^a	445 ± 51	307 ± 3^a	1.8 ± 0.2	3.9 ± 0.2^a	2.3	1.4	3.4
Lys	24 ± 2	14.6 ± 0.6^a	38 ± 1	29 ± 2^a	0.2 ± 0.02	0.3 ± 0.02^a	0.6	1.3	0.8
Met	4.4 ± 0.3	7.8 ± 0.7^a	31 ± 2	8.1 ± 0.6^a	0.1 ± 0.0	0.1 ± 0.01^a	1.8	3.9	6.9
Thr	307 ± 33	377 ± 38	984 ± 37	624 ± 60^a	4.5 ± 0.2	6.8 ± 0.3^a	1.2	1.6	1.9
Val	29 ± 1	35 ± 3^a	122 ± 9	42 ± 4^a	0.6 ± 0.03	0.5 ± 0.02^a	1.2	2.9	3.5
Ile	8.1 ± 0.7	7.0 ± 0.4	20 ± 1	14 ± 1^a	0.1 ± 0.01	0.2 ± 0.01^a	0.9	1.4	1.2
Leu	35 ± 6	35 ± 3	124 ± 10	85 ± 9^a	0.6 ± 0.1	0.8 ± 0.06^a	1.0	1.5	1.4
Phe	13.6 ± 0.7	12.2 ± 0.8	4,654 ± 373	400 ± 98^a	21.0 ± 1.0	6.7 ± 1.3^a	0.9	11.6	10.5
Tyr	7.6 ± 0.6	4.8 ± 0.6^a	91 ± 5	17 ± 3^a	0.4 ± 0.02	0.2 ± 0.01^a	0.6	5.3	3.3
Trp	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
Total	4,559 ± 229	8,356 ± 1,134^a	22,328 ± 2,009	7,819 ± 344^a	100	100	1.8	2.9	5.2

Ws-2 and pig1-1 seeds had been germinated and grown at a PFD of 150 μmol m⁻² s⁻¹ on half-strength MS, 0.5% Suc with or without 2 mm Phe prior to harvesting whole plantlets after 14 d. From left to right: The four columns on the left represent absolute free amino acid contents in nmol g fw⁻¹, while the next two columns give the relative amino acid abundance for Phe-replete grown plantlets in mol %. Based on the absolute values shown, ratios between free amino acid contents in pig1-1 and the wild type were calculated (right three columns, ratios are either given as pig1-1 to Ws-2 or Ws-2 to pig1-1 to indicate an increase or decrease, respectively). n.d., Not determined. ⁺, In comparison to the wild-type Ws-2. *, The repression ratio (rr) expresses how much more the content of any given amino acid (aa) is diminished in pig1-1 compared to the wild type upon feeding 2 mm Phe. It was calculated as rr = ([aa]pig on soil/[aa]Ws on soil) × ([aa]Ws on Phe/[aa]pig on Phe). The data represent the mean values ± sd of five independent replicates (n). The data were tested for significant differences between the wild type and the mutants using the Welsh method.

Significant differences with P < 0.01 are indicated.