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. 2017 Jan 13;10:310. doi: 10.3389/fncel.2016.00310

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Copyright © 2017 Sutherland, Mathews, Mao, Nguyen and Gorrie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative high power fluorescent images of nestin staining (red) at the lesion edge in (A) Adults, (B) Juveniles, and (C) Infants. These represent the areas denoted by the squares in low power images of one side of the transverse spinal cord shown in (D) Adults, (E) Juveniles, and (F) Infants 1 week post injury. The lesion is denoted by ^*, the central canal is indicated by # where it is intact, and the midline by the blue line. (G) Histogram of the intensity of nestin staining (mean grayscale value) indicating endogenous neural progenitor cells and processes around the edge of the lesion or cavity at 24 h, 1, 2, and 6 weeks post-injury. Using two-way ANOVA there was a significant difference (P < 0.0001) in both main effects, age and survival time. Significant differences (P < 0.005) are shown between groups based on Bonferroni's post-hoc test.