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. 2004 Oct;136(2):3080–3094. doi: 10.1104/pp.104.045369

Figure 2.

Figure 2.

PATL1 antibody production and characterization. The Arabidopsis PATL1 coding region was expressed as a GST-fusion in E. coli and purified for biochemical analysis and antibody production. A, SDS-PAGE of uninduced (lane 1) and induced (lane 2) E. coli extracts, purified GST-patellin (lane 3), and cleaved fusion protein (lane 4). Molecular mass markers are shown at left. B, Immunoblots of Arabidopsis root total protein extracts (10 μg/lane) probed with PATL1 antisera raised against full-length protein (lane 1) or the GOLD domain (lane 2). C, Immunoblots of PATL1 and PATL2-GST fusion proteins probed with PATL1 antibody. D, Immunoblots of Arabidopsis organ extracts (10 μg/lane) probed with PATL1 or actin antibodies.