Figure 6.

Structure of the GRV2 locus. A, The region of chromosome II containing the GRV2 gene was spanned by bacterial artificial chromosomes F12C20 and T20P8 (www.arabidopsis.org). A cosmid contig spanning the GRV2 gene is shown as overlapping lines beneath The Arabidopsis Information Resource annotation of the region. The three genes in the complementing cosmid, pRAM011, are At2g26870 (a), At2g26880 (b), and At2g26890 (c). B, The intron-exon structure of the GRV2 gene (At2g26890) is shown as lines and boxes, respectively. The locations of various mutations are indicated by lines and arrowheads. grv2-1 has an 85-residue truncation, grv2-4 has a frame shift resulting in a stop codon in exon 11, and GT1669 and SALK 067162 are T-DNA insertions. A schematic drawing of the GRV2 protein is shown below the transcript schematic. White boxes represent IWN repeat domains, and the black circle represents the J domain. The expressed sequence tag, RZL04ho7, and the reverse transcription-PCR products that were sequenced to determine the intron-exon structure (α, β, γ, δ, ɛ, ξ, η, θ, ι) are shown beneath the protein structure.