Figure 7.

(a) Yeast two-hybrid system-based screening of ginger CDPK1 interactors using the ginger cDNA library. Yeast cells on QDO/A plates (quadruple dropout medium i.e., selection medium with all essential amino acids except four amino acids (adenine, histidine, tryptophan, and leucine) and supplemented with Aureobasidin A). (b) PCR amplification using AD specific primers on ZoCDPK1-ginger Y2H library interacting colonies on selection medium. The selection was done on quadruple dropout medium lacking adenine, histidine, tryptophan, and leucine and supplemented with Aureobasidin A.