Table 1.

Precision assay of the proposed fluorescent ELISA

Sample matrix	OTA added (µg kg−1)	OTA recovered (µg kg−1)	Recovery (%)	CV (%)
Intra-assay (n = 3)a
Wheat	4	3.92 ± 0.29	98.00	7.44
	10	10.22 ± 1.81	102.20	17.67
	20	18.75 ± 1.05	93.75	5.58
	40	40.54 ± 3.07	101.35	7.58
Corn	4	4.09 ± 0.63	102.25	15.35
	10	10.23 ± 0.85	102.30	8.30
	20	19.26 ± 3.00	96.30	15.58
	40	37.50 ± 2.70	93.75	7.21
Rice	4	3.48 ± 0.27	87.00	7.70
	10	9.12 ± 1.20	91.20	13.12
	20	17.72 ± 0.88	88.60	4.96
	40	34.51 ± 2.55	86.28	7.40
Inter-assay (n = 6)b
Wheat	4	4.11 ± 0.43	102.75	10.49
	10	9.56 ± 1.48	95.60	15.53
	20	18.94 ± 1.08	94.70	5.69
	40	41.09 ± 2.72	102.73	6.61
Corn	4	4.19 ± 0.43	104.75	10.32
	10	9.79 ± 1.06	97.90	10.86
	20	19.21 ± 2.23	96.05	11.58
	40	38.83 ± 2.45	97.08	6.30
Rice	4	3.89 ± 0.71	97.25	18.36
	10	9.09 ± 0.92	90.90	10.09
	20	18.62 ± 1.77	93.10	9.51
	40	36.57 ± 3.37	91.43	9.21

^aThe assay was performed in three replicates on the same day.

^bThe assays were carried out on six consecutive days.