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. 2004 Oct;136(2):3134–3147. doi: 10.1104/pp.104.046169

Figure 6.

Figure 6.

PCR analysis and genome location of T-DNA insertion in sto1/nced3 mutants. A, Secondary TAIL-PCR product (lane 2) and shift of the tertiary PCR product (lane 3) in sto1. B, Correct genomic integration of the T-DNA insertion was verified by diagnostic PCR; lanes 1 (marker); 2 (DNA template, wild type; primers, T-DNA LB [3′] and STO1-specific primer [5′]); 3 (DNA template, sto1; primers, T-DNA LB [3′] and STO1-specific primer [5′]); 4 (DNA template, wild type; primers, sto1-specific primer [3′] and STO1-specific primer [5′]); 5 (DNA template, sto1; primers, STO1-specific primer [3′] and STO1-specific primer [5′]). Oligonucleotide sequences are reported in Table V. C, Physical map of the sto1 locus and insertion site of the T-DNA. Solid line represents fragment of the bacterial artificial chromosome clone MOA2.4. Black box indicates the coding region of the gene; arrow indicates the predicted transcription direction.