Figure 7.

NCED3 transcript abundance is increased by NaCl treatment and is reduced in sto1/nced3 mutant plants. Ten micrograms of total RNA were isolated from 21-d-old sto1/nced3 mutant and wild-type plants that were germinated and grown on 145 mm NaCl, separated on a denaturing formaldehyde-agarose gel, and blotted onto nylon membrane. The membrane-bound RNA was hybridized with DIG-labeled DNA probe (Roche, Indianapolis). The probe was produced by PCR reaction using the primers listed in Table V.