Skip to main content
. 2004 Oct;136(2):3191–3197. doi: 10.1104/pp.104.048967

Figure 2.

Figure 2.

Analysis of Pcg12::GUS and endogenous MtENOD20 expression in transgenic M. truncatula plants inoculated with wild-type (Rm2011), mutant (nodA) S. meliloti, purified Nod factors, or water (control) 2 and 7 dai. A, Detail of a root 7 dai with S. meliloti Rm2011 showing GUS activity in a deformed root hair and the adjacent cortical cells. B, Transcriptional activation of MtENOD20 expression 2 and 7 dai. Inoculated roots were harvested, total RNA extracted, and MtENOD20 expression analyzed by RT-PCR. MtENOD20 primers are situated on both sides of the single MtENOD20 intron (Vernoud et al., 1999) so that cDNA and genomic amplification products can be separated. Amplification of a constitutive actin cDNA (MtActin; Cohn et al., 2001) was used as a positive control. A, Bar = 100 μm.