Fig. 6.

Advax alters gene expression of IL-1β, CLRs and TNF-α signaling pathways. Whole organ (lung; LG, liver; LV, spleen; SP, kidney; KD, lymph node; LN) transcriptome of 6 h after Advax administration (i.d. or i.p.) alone was obtained by Affimetrix GeneChip (n = 3). (a) Only selected gene probes (FC > 2 and PA = 1) are displayed. (b) Advax-responding cell population analysis was performed (see Materials & Methods; cell population analysis). The left hemispheres of the figure denote the ten immune cell types and right hemispheres denote the samples. The ribbons in the inner circle denote the cell type score of each sample. The colors in the middle layer rings represent either cell types or samples. The outermost ring denotes the percentage of total contribution for every factor (cell type or samples) viewed from their counterpart factor. For example in SP.ip, neutrophils constitute around 30% of the cell population. (c) IPA upstream regulator analysis of Advax-induced genes in the SP was performed. (see Materials & Methods; upstream regulator analysis in IPA). FC: Fold Change, PA: Presence and Absence. (see Materials & Methods; microarray analysis).