Fig. 2.
Effect of LDLs on normal lymphocytes and leukemic cell-lines. A–C. Purified normal PBMCs (1 × 106 cells/ml) were stimulated with IL-2 and Resiquimod (2S) and cultured for 18 h with or without LDL (0.5 mM). Nile Red- (A) (n = 4) and PFO (B)-staining (n = 5) were measured by flow cytometry. C. Changes in pSTAT3 levels were determined by immunoblotting with total STAT3 protein levels used as a loading control (n = 5). D. Oncomine analysis of the Haferlach data base comparing 74 normal PBMC and 448 CLL samples indicates significantly lower SORL1 expression in CLL cells compared to PBMCs and other hematologic malignancies. E–F. Vector control and PPARDhi Daudi were cultured in RPMI-1640 ± LDL (0.5 mM). Cells were counted after 4 days (E) and pSTAT3 levels measured after 18 h (F). G. Cholesterol levels in control and PPARDhi Daudi cells in exponential growth in RPMI + 5% FCS were measured by Amplex red staining. *p < 0.05; **p < 0.01; ns = not significant.