Fig. 3.

Effect of LDLs on cytokine-signaling in activated CLL cells in vitro. Purified CLL cells were cultured in lipid-poor conditions with or without IL-2 and Resiquimod and with or without addition of LDLs (0.5 mM) in the presence or absence of IL-6 antibodies (10 ng/ml), IL-10 antibodies (10 ng/ml) or Ruxolitinib (Rux) (500 nm). A. After 18 h, phospho-STAT3 levels were determined in 4 patient samples by immunoblotting and densitometry and normalized to the results for β-actin. The averages and standard deviations of the relative densitometric values are indicated in the graph and a representative immunoblot is shown. B, C, D. After 48 h, IL10 levels in the culture supernatants were measured by ELISAs (n = 5) (B), IL10 mRNA was measured by quantitative RT-PCR for Pt.117 (C), and mean fluorescence intensities of IL10-receptor (IL10R)-staining were determined by flow cytometry for 5 patient samples (D). Averages and standard deviations are shown. *p < 0.05; **p < 0.01; ns, non significant.