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. 2016 Dec 21;15:137–149. doi: 10.1016/j.ebiom.2016.12.010

Fig. 2.

Fig. 2.

Lyn inhibited mucus hypersecretion and the ER stress-associated pathway in allergic airway inflammation disease. (a) Representative PAS staining of the lung tissue of WT mice and Lyn-TG mice. Representative immunohistochemical staining of Muc5ac in the lung tissue of WT mice and Lyn-TG mice (original magnification: 200 ×). (b–c) The goblet cell percentage was quantified in 10 random fields. The fluorescence intensity of Muc5ac was quantified per micrometer in 10 random fields. (d) RT-PCR of Muc5ac mRNA expression. (e) Representative Western blots of phospho-PI3K p85α, PI3K p85α, phospho-Akt1, Akt1, phospho-NFκB p65 (Ser536), NFκB p65, Lyn, β-actin, BIP, CHOP, and phospho-Lyn (Tyr416) in the lung tissue. (f–i) Relative changes in the density of phospho-Lyn and β-actin, phospho-PI3K p85α and PI3K p85α, phospho-Akt1 and Akt1, and phospho-NFκB p65 (Ser536) and NFκB p65, as measured by Western blot. Data are representative of three experiments. Bars represent the mean ± s.d (n = 8 each group, one-way ANOVA with Tukey-Kramer post-test).