Fig. 4.

Lyn regulated IL-13-induced NFκB activity in vitro. (a) NFκB nuclear translocation was assessed by immunofluorescence staining with the NFκB p65 antibody. Nuclei were counterstained with DAPI (blue), TRICT-conjugated secondary Abs for NFκB (red); eGFP or Lyn-eGFP (green). (b) Representative Western blot of NFκB in the cytosolic or nuclear protein extract. (c) Relative change in density of NFκB in the nuclear protein extracts, as measured by Western blot. Data are representative of three experiments. Bars represent the mean ± s.d (n = 8 each group, one-way ANOVA with Tukey-Kramer post-test).