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PPO inhibits postharvest proteolysis in an o-diphenol-dependent manner. Amino acid release during a 4-h incubation at 37°C was used to measure proteolysis in extracts of control or PPO1-expressing alfalfa as indicated in the presence (+CA) or absence (−CA) of 3 mm caffeic acid. Amino acid release is expressed as μmol mg−1 extract protein, and values are the average of three experiments ±se.
