Table I.
Enzyme activity in transgenic alfalfa expressing red clover PPO cDNAs
| Tissue Source | No. of Plants PPO Positivea/Total | PPO Activity
|
|
|---|---|---|---|
| Range | Median ± MADb | ||
| nkat mg−1 | |||
| PPO1-alfalfa | 4/4 | 1.45–4.90 | 3.57 ± 1.18 |
| PPO2-alfalfa | 21/27 | 0–0.38 | 0.10 ± 0.07 |
| PPO3-alfalfa | 12/13 | 0.02–4.18 | 0.67 ± 0.53 |
| Red cloverc | – | 6.28–72.81 | 41.41 ± 27.47 |
| Control alfalfac | – | 0–0.02 | 0.02 ± 0.00 |
a
PPO-positive plants are defined as those having PPO activities above the measured range for control alfalfa.
b
Median absolute deviation.
c
Values represent multiple measurements made on a single genotype (red clover) or transformant (control alfalfa). Average PPO activity ±se measured was 34.54 ± 11.96 and 0.02 ± 0.00 nkat mg−1 for red clover and control alfalfa, respectively.