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Kinetic parameters for NtOSAK
| Substrate | Km | Vmax |
|---|---|---|
| μm | nmol/min/mg | |
| ATP | 14.4 ± 2.0 | 573 ± 34 |
| MBP | 23.2 ± 4.8 | 220 ± 18 |
| Casein | 26 ± 3 | 130 ± 6 |
| GST-ACC | 17 ± 6 | 18.1 ± 3.4 |
| SAMS | 125 ± 19 | 399 ± 27 |
| AMARA | 63 ± 10 | 300 ± 19 |
Km and maximal velocity (Vmax) for protein and peptide substrates, as well as for ATP, were determined for three independent experiments. Parameters were estimated by fitting of data directly to the Michaelis-Menten equation using GraphPad Prism 3.0, and are quoted ±se.
