Table II.
Genes whose salt-induced expression was enhanced with the induction factor higher than 2.0 after salt treatment with 684 mM NaCl (4%, w/v) for 24 h or 5 d
24 h
|
5 d
|
|||||||
---|---|---|---|---|---|---|---|---|
ORFa | Gene | Function | Adb | Ind-Factorc | Stdevd | Groupe | Ind-Factorf | Stdevg |
sll1862h | Unknowni | a | 49.87 | 4.51 | 3 | 9.55 | 1.65 | |
sll1863h | Unknown | a | 25.44 | 1.17 | 3 | 11.37 | 1.04 | |
sll1566h | ggpS | Glucosylglycerol Phosphate synthase | b | 7.56 | 0.36 | 3 | 3.97 | 1.18 |
slr0765h | mscS | Mechano-sensitive channel | c | 6.82 | 0.07 | 4 | 3.42 | 0.80 |
slr0747h | ggtA | ATP-binding subunit of the osmolyte transporter | d | 5.19 | 0.30 | 3 | 2.79 | 0.02 |
sll0528 | Unknown | 5.04 | 0.34 | 3 | 1.59 | 0.30 | ||
slr1672h | glpK | Glycerol kinase | b | 5.01 | 0.35 | 3 | 2.74 | 0.71 |
slr0786h | map | Methionine aminopeptidase | 4.01 | 0.83 | 3 | 2.33 | 0.15 | |
slr0903 | moaE | Molybdopterin (MPT) converting factor, subunit 2 | 3.73 | 0.20 | 3 | 2.58 | 0.61 | |
sll1085h | glpD | Glycerol-3-phosphate dehydrogenase | b | 3.53 | 0.55 | 3 | 2.66 | 0.28 |
slr1894h | Probable DNA-binding stress protein | 3.22 | 0.43 | 3 | 2.51 | 0.24 | ||
slr1687 | Probable phycobilin lyase | e | 3.14 | 0.12 | 3 | 1.05 | 0.21 | |
ssr2595 | hliB | High light-inducible protein | h | 3.01 | 0.36 | 3 | 0.85 | 0.02 |
slr1670h | Unknown | b | 2.89 | 0.08 | 3 | 2.00 | 0.62 | |
sll1898 | Probably involved in cytochrome aa3 oxidase assembly | f | 2.89 | 0.02 | 4 | 2.06 | 0.14 | |
slr0898 | nirA | Ferredoxin-nitrite reductase | 2.80 | 0.20 | 3 | 0.82 | 0.13 | |
slr0746h | stpA | Glucosylgycerol phosphate phosphatase | d | 2.75 | 0.54 | 3 | 2.24 | 0.08 |
slr0529 | ggtB | Substrate-binding protein of the osmolyte transporter | g | 2.63 | 0.13 | 3 | 1.48 | 0.28 |
sll1071 | Unknown | 2.61 | 0.67 | * | 0.99 | 0.04 | ||
slr1673 | spoU | rRNA methylase | b | 2.48 | 0.05 | 3 | 1.74 | 0.36 |
ssl3044 | Probable ferredoxin | e | 2.44 | 0.68 | 3 | 0.95 | 0.01 | |
slr1674h | Unknown | b | 2.41 | 0.20 | 3 | 2.94 | 0.22 | |
slr0193 | rbp3 | RNA-binding protein | 2.24 | 0.51 | 3 | 0.83 | 0.03 | |
sll1330 | rre37 | Response regulator | 2.23 | 0.13 | 3 | 1.89 | 0.21 | |
sll0745 | pfkA | Phosphofructokinase | c | 2.23 | 0.24 | 3 | 1.41 | 0.00 |
sll0018 | cbbA | Fructose-1,6-bisphosphate aldolase | 2.18 | 0.58 | * | 0.88 | 0.04 | |
sll0556 | nhaS6 | Na+/H+ antiporter | 2.16 | 0.32 | 2 | 0.89 | 0.01 | |
slr1938 | Putative translation initiation factor EIF-2b subnuit 1 | 2.16 | 0.01 | 3 | 1.64 | 0.21 | ||
sll1980 | trxA | Thiol-disulfide interchange protein (thioredoxin) | 2.16 | 0.09 | 4 | 1.48 | 0.23 | |
sll0807 | cfxE | Pentose-5-phosphate-3-epimerase | 2.14 | 0.07 | * | 2.15 | 0.12 | |
slr1544 | Unknown | h | 2.14 | 0.17 | 2 | 0.99 | 0.27 | |
sll0430 | htpG | Heat shock protein | 2.13 | 0.07 | 3 | 1.36 | 0.11 | |
slr0530 | ggtC | Permease subunit of the osmolyte transporter | g | 2.13 | 0.21 | 3 | 1.42 | 0.20 |
sll1899 | ctaB | Cytochrome c oxidase folding protein | f | 2.09 | 0.24 | 4 | 2.02 | 0.59 |
slr1932 | Unknown | a | 2.09 | 0.05 | 2 | 1.36 | 0.10 | |
slr1549 | Polypeptide deformylase | 2.09 | 0.04 | 3 | 1.35 | 0.06 | ||
sll0828 | nylA | 6-Aminohexanoate-cyclic-dimer hydrolase | 2.07 | 0.47 | 1 | 1.40 | 0.01 | |
ssl2542 | hilA | High light-inducible protein | 2.05 | 0.14 | 3 | 0.92 | 0.16 | |
sll1653 | menG | 2-Phytyl-1,4-benzoquinone methyltransferase | 2.04 | 0.41 | 2 | 1.01 | 0.16 |
Experimental conditions for salt treatment and DNA-microarray analysis are described in “Materials and Methods.”
Open reading frame (ORF) designations according to Kaneko et al. (1996).
Adjacent genes (Ad) on the chromosome are indicated by identical letters.
Induction factor for each gene.
Experimental deviation in a typical experiment.
Group number according to Figure 1 (*, genes maximally expressed 24 h after salt addition).
Induction factor for each gene in cells acclimated for 5 days to 684 mM NaCl.
Standard deviation from 3 independent experiments.
ORFs increasingly expressed in cells acclimated to increasing salt concentrations.
ORFs for proteins of unknown function, so-called hypothetical proteins.