Effect of RU486 on total food intake in Dahomey males. Estimated consumption over 24 hours, assessed by radioisotope labeling, after (A) 0 or (B) 10 days of habituation on the indicated diet. (C) Total consumption over 24 hours of the indicated diet, assessed by CAFE assay. (D) Estimated consumption over 24 hours of 5% YE diet, assessed by radioisotope labeling. Diets were supplemented with 200 µM RU486 (+) or vehicle (−). Data shown are means ± SEM. n = number of vials or CAFE chambers, superimposed on each bar. Significant differences between RU486- and vehicle-supplemented diet are shown (Student’s t test or Mann–Whitney rank-sum test: *p < .05; ***p < .001). CAFE = capillary feeder; YE = yeast extract.