Figure 4.
Ribosomal protein synthesis stimulated by UV-B irradiation. Ribosomal proteins were resolved by 10% to 20% SDS-PAGE after in vivo [35S]Met labeling, visualized by autoradiography (A) and staining with Coomassie Blue (B) following the 6-h UV-B treatment (+UV-B) or in control conditions without UV-B (−UV-B). C, Quantification of radioactive labeled proteins by densitometry of the autoradiograph. The experiment was repeated three times and normalized to equal amounts of ribosomal protein. The molecular mass of marker proteins is indicated in the right of B.